Breakit:
Don't know if you have already done something about your climate control system. In answer to your questions:
We had a service contract with Climatec, which was the controls subcontractor when the building was built. There are of course other nationally-known firms that will have the knowledge to "do stuff" in this area. Your typical 9-person local HVAC company may not.
The building we built was 60,000 sq ft, in the Southwest, using outdoor chillers with pumps for circulating chilled water to a total of 7 air handlers inside (3 for cleanroom, 4 for rest of building). The air handlers, driven by VFDs, responded to the total load as represented by the individual thermostat settings reported to the controller interface. This is all just a little background info. Typically the air handlers always ran at some speed, so there was always some positive pressure in the HVAC duct system.
The specific gear for the tight control was PID control on the damper for air flow from the air handler (basically controls how cool it gets). In addition, there was a reheat coil in the damper assy to heat the air supply if/when required; the valve also was driven by the PID control. Tuning the room response took some time, probably a few weeks of observation, adjustment, observation, blah blah...It helps to NOT have the airflow directly hitting the sensor... If you engage a knowledgable company, they will be able to calculate the airflow required, and thereby the ducting cross-sections, number of vents, and the controls required for the space. The important thing is to have a good idea of what it is you want to achieve, and what your fallback is when you get the price....
The room in which we first did this was about 150 sq ft with about 9 ft ceiling, and there were 2 computer systems running most of the time, along with test interferometers for optical component checking. Without the control system, the general building systems would cause several degrees of temp swing, which gives some lousy results with tests where you are looking at fractional-nanometer results for process control ... The close temp control really helped.
As other posters have noted, if you are building a room from scratch, for this application, having no walls that are exterior is a big plus. Cost-wise, the gear to ADD this control to an already-existing basic HVAC system with the infrastructure already there was about $3500 including the plumbing run (~40') for the line to the hot water supply, some software adjustments, a new damper assy, and a good bit of work in a small room with a manlift at about 15' off the floor through the dropped ceiling. If your stuff is more accessible, some of the work could be less expensive.